Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
- (1)
- (1)
- (3)
- (1)
- (108)
- (1)
- (2)
- (1)
- (2)
- (2)
- (69)
- (2)
- (1)
- (1)
- (1)
- (1)
- (10)
- (3)
- (2)
- (10)
- (6)
- (1)
- (4)
- (2)
- (10)
- (1)
- (5)
- (5)
- (110)
- (107)
- (1)
- (1)
- (1)
- (1)
- (1)
- (11)
- (2)
- (7)
- (1)
- (1)
- (1)
- (2)
- (6)
- (8)
- (23)
- (1)
- (12)
- (22)
- (1)
- (4)
- (1)
- (12)
- (1)
- (3)
- (1)
- (53)
- (6)
- (5)
- (34)
- (129)
- (2)
- (1)
- (26)
- (2)
- (1)
- (6)
- (1)
- (1)
- (3)
- (19)
- (2)
- (1)
- (26)
- (1)
- (1)
- (6)
- (7)
- (3)
- (8)
- (1)
- (86)
- (148)
- (8)
- (6)
- (10)
- (1)
- (1)
- (4)
- (1)
- (1)
- (1)
- (1)
- (1)
- (2)
- (2)
- (17)
- (143)
- (6)
- (3)
- (1)
- (3)
- (3)
- (7)
- (5)
- (3)
- (8)
- (1)
- (598)
- (5)
- (4)
- (4)
- (114)
- (23)
- (12)
- (48)
- (5)
- (4)
- (2)
- (5)
- (125)
- (2)
- (13)
- (10)
- (3)
- (14)
- (49)
- (1)
- (2)
- (31)
- (13)
- (26)
- (2)
- (7)
- (9)
- (9)
- (23)
- (45)
- (5)
- (41)
- (11)
- (5)
- (13)
- (1)
- (3)
- (2)
- (3)
- (1)
- (9)
- (1)
- (94)
- (3)
- (4)
- (4)
- (3)
- (3)
- (3)
- (10)
- (3)
- (31)
- (4)
- (3)
- (9)
- (3)
- (45)
- (1)
- (3)
- (1)
- (4)
- (1)
- (1)
- (2)
- (1)
- (1)
- (3)
- (5)
- (1)
- (4)
- (3)
- (5)
- (1)
- (10)
- (10)
- (6)
- (4)
- (2)
- (5)
- (3)
- (2)
- (5)
- (10)
- (1)
- (10)
- (1)
- (2)
- (3)
- (2)
- (2)
- (2)
- (5)
- (7)
- (1)
- (2)
- (10)
- (4)
- (2)
- (1)
- (1)
- (4)
- (3)
- (3)
- (1)
- (2)
- (1)
- (3)
- (3)
- (2)
- (13)
- (4)
- (1)
- (4)
- (3)
- (2)
- (2)
- (2)
- (1)
- (4)
- (2)
- (4)
- (3)
- (10)
- (1)
- (1)
- (1)
- (1)
- (14)
- (1)
- (38)
- (2)
- (1)
- (2)
- (9)
- (2)
- (3)
- (8)
- (2)
- (1)
- (2)
- (3)
- (1)
- (1)
- (1)
- (32)
- (1)
- (5)
- (8)
- (2)
- (14)
- (5)
- (1)
- (3)
- (5)
- (1)
- (23)
- (1)
- (62)
- (11)
- (1)
- (43)
- (1)
- (11)
- (2)
- (1)
- (2)
- (1)
- (9)
- (1)
- (38)
- (1)
- (1)
- (7)
- (1)
- (1)
- (5)
- (1)
- (2)
- (1)
- (1)
- (21)
- (5)
- (3)
- (1)
- (17)
- (3)
- (1)
- (4)
- (4)
- (1)
- (6)
- (1)
- (4)
- (77)
- (93)
- (3)
- (6)
- (21)
- (53)
- (1)
- (72)
- (1)
- (29)
- (1)
- (1)
- (49)
- (15)
- (5)
- (2)
- (29)
- (1)
- (1)
- (10)
- (5)
- (5)
- (4)
- (2)
- (1)
- (7)
- (12)
- (2)
- (7)
Filtered Search Results
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 7.2
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
MilliporeSigma™ PBS Tablets, Calbiochem™,
Dissolving one tablet in 1 liter of deionized H2O yields 140mM NaCl, 10mM phosphate buffer, and 3mM KCl
Gibco™ DPBS, powder, calcium, magnesium
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Includes | No Sodium Pyruvate |
|---|---|
| Physical Form | Powder |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | DPBS |
| For Use With (Application) | Mammalian Cell Culture |
| Solution Type | Dulbecco's Phosphate Buffered Saline |
| Product Line | Gibco |
| Molecular Weight (g/mol) | 89.14 |
|---|---|
| Color | Colorless |
| Physical Form | Liquid |
| SMILES | CC(C)(N)CO |
| Concentration | 0.2 M |
| InChI Key | CBTVGIZVANVGBH-UHFFFAOYSA-N |
| CAS | 124-68-5 |
| MDL Number | MFCD00008051 |
| pH | 9.5 |
| Synonym | 2-Amino-2-methyl-1-propanol |
| Recommended Storage | Keep cold |
| IUPAC Name | 2-amino-2-methylpropan-1-ol |
| Molecular Formula | C4H11NO |
| Odor | Odorless |
Thermo Scientific Chemicals TRIS, 0.5M buffer soln., pH 8.8
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Invitrogen™ PBS (Phosphate-Buffered Saline) Tablets
Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cellular applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Gibco™ DPBS, calcium, magnesium, glucose, pyruvate
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Includes | Sodium Pyruvate |
|---|---|
| Physical Form | Liquid |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | DPBS |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
| Sterility | Sterile-filtered |
| Solution Type | Dulbecco's Phosphate Buffered Saline |
| Product Line | Gibco |
| Color | Red |
|---|---|
| Physical Form | Liquid |
| Recommended Storage | Ambient temperatures |
MilliporeSigma™ MOPS, Free Acid, Molecular Biology Grade, Calbiochem™,
CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 3-(N-Morpholino)propanesulfonic Acid PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 4-(3-sulfonatopropyl)morpholin-4-ium SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
| PubChem CID | 70807 |
|---|---|
| CAS | 1132-61-2 |
| Molecular Weight (g/mol) | 209.26 |
| ChEBI | CHEBI:44115 |
| MDL Number | MFCD00006183 |
| SMILES | [O-]S(=O)(=O)CCC[NH+]1CCOCC1 |
| Synonym | 3-(N-Morpholino)propanesulfonic Acid |
| IUPAC Name | 4-(3-sulfonatopropyl)morpholin-4-ium |
| InChI Key | DVLFYONBTKHTER-UHFFFAOYSA-N |
| Molecular Formula | C7H15NO4S |
Corning™ Mediatech™ Cell Culture Phosphate Buffered Saline (10X)
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
With low endotoxin content
| Name Note | low endotoxin |
|---|---|
| MDL Number | MFCD00132476 |
| Color | Colorless |
| Physical Form | Liquid |
| Chemical Name or Material | TRIS-buffered saline (10X, pH 7.4) |
| TSCA | Yes |
| Recommended Storage | Ambient temperatures |
| Physical Form | Liquid |
|---|---|
| pH | 6.5 |
| Chemical Name or Material | MNE buffer |
| TSCA | Yes |
| Recommended Storage | Ambient temperatures |
Gibco™ DPBS (10X), calcium, magnesium
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
MilliporeSigma™ PIPES, Sodium Salt, Molecular biology grade, OmniPur™, Calbiochem™,
CAS: 100037-69-2 Molecular Formula: C16H33N4Na3O12S4 Molecular Weight (g/mol): 670.665 InChI Key: FPDACHMSOMILQI-UHFFFAOYSA-K PubChem CID: 3086462 IUPAC Name: trisodium;2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonate;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)[O-].C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+].[Na+]
| PubChem CID | 3086462 |
|---|---|
| CAS | 100037-69-2 |
| Molecular Weight (g/mol) | 670.665 |
| SMILES | C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)[O-].C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+].[Na+] |
| IUPAC Name | trisodium;2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonate;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate |
| InChI Key | FPDACHMSOMILQI-UHFFFAOYSA-K |
| Molecular Formula | C16H33N4Na3O12S4 |